Year: 2017 | Month: December | Volume 7 | Issue 2
In-vitro Screening and Characterisation of Conjugated Linoleic Acid Producing Lactic Acid Bacteria
Abstract:
Conjugated Linoleic Acid (CLA) isomers have attracted significant attention due to their important functional traits with plenty of health prospects to humans. Many Lactic Acid Bacteria (LAB) demonstrate the ability to produce CLA isomers C18:2 cis-9, trans-11 and C18:2 trans-10, cis-12 from the Linoleic Acid (LA) in-vitro. In the present study, 16 LAB with ability to produce CLA were isolated from diverse ecosystem. Three LAB isolates, CMLAB2, BMLAB1 and PMLAB1 had shown their potential to convert in-vitro free LA to CLA. The FAME extracts of CLA produced in fermentation experiments by these isolates were studied. The isolates CMLAB2, BMLAB1 and P2MLAB1 were found to produce 21.21; 14.68; 6.35 μg/ml of CLA respectively. Among these isolates, CMLAB2 isolate was found to produce physiologically important and most studied c9, t11CLA and t10, c12 CLA isomers in significant amount in-vitro. Upon characterization of this isolate, it was identified as a species of Lactobacilli.
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